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Nuclease Free Water In Pcr

November 29, 2021

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If i used my dna at full concentration, my pcr reactions often do not work. Can ultra pure water (dnase and rnase free) serve the purpose of nuclease free water?.

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Only if i dilute the dna at least 1:5 in water, do i get a signal.

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Nuclease free water in pcr. Nuclease free water is used in order to dilute the concentration of the reagents to the proper final concentration. As commercially available nuclease free. The 2 ml vial provides convenient use.

Also use of nuclease free water helps avoid dna degradation by nucleases as well as interference of the pcr reaction by ions which could be present in otherwise not nuclease free deionized water. Ibi pcr grade water is designed specifically for pcr work due to its small volume packaging. So, i keep my dna stocks in te.

Each lot tested for nuclease contamination. Pcr, cdna synthesis, or qpcr). Available in 25 ml and 100 ml sizes.

And for pcr, i make 1:5 to 1:25 dilution (in sterile distilled water) of my dna as working templates. No toxic agents, such as depc, are used in production, so as to avoid inhibition in enzymatic reactions. We must use nuclease free water in pcr reaction so as to prevent degredation of dna.

Even purified water can have a high ph and minerals that can interfere with reactions that require specific salt and ph conditions. Ambion offers three different grades of high quality water: Nuclease free water is used in order to dilute the concentration of the reagents to the proper final.

The presence of nucleases such as dnase and rnase in water can degrade precious molecular samples and even ruin experiments. Nuclease free water (depc free) has not been treated with depc and is certified for all molecular biology applications requiring nuclease and nucleic acid free water. For pcr work and plasmid dna storage which water is best to use?

Ideal for the preparation of reagents and for use in enzymatic reactions.

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